HPLC, or High Performance Liquid Chromatography, is a gold-standard analytical technique used worldwide for accurate separation, identification, and quantification of compounds in complex mixtures. This in-depth guide from Zodiac Life Sciences explores the fundamentals of HPLC, its working principles, instrumentation, column types, and key applications across pharma, biotech, and research industries. Whether you're new to HPLC or looking to refine your skills, this article provides expert knowledge to boost your analytical capabilities and results.